Prevalence and Risks of Fascioliasis among Adult Cohorts in Binh Dinh and Quang Ngai Provinces-Central Viet Nam

Quy Minh Tran, Heather Yeatman, V Flood, Chuong NC, Tuan BV

Abstract


Fascioliasis (liver fluke disease) has raised significant public health concerns in the 15 regional provinces of Central Vietnam, accounting for 93% of the national incidence of the disease. No control measures to date have proven effective. Annual reports show increasing incidence of fascioliasis but they are incomplete. This cross-sectional study was conducted to identify the prevalence of fascioliasis and to describe its associated risks in three communes in Central Vietnam. 500 human blood samples were examined (ELISA); and a survey of knowledge, attitude and practice (KAP) was conducted for 600 randomly selected adults per commune. The findings suggest that overall seroprevalence was 7.75% (95% CI 6.54-9.16%). Among the infected cases, people aged from 18-59 years (85.6%) and farmers (68.0%) accounted for majority of infection. Less than half of participants in all three communes (24.6% - 46.0%) knew the causes of fascioliasis; and considerable proportions ate improperly boiled vegetables (28.2-33.8%), drank unboiled water (23.5-42.5%), and did not own a household toilet (14.2-20.5%). Relatively high prevalence and risks of fascioliasis were found in Central Vietnam, supporting the need for comprehensive intervention measures including selective treatment, health education, and multisectoral approaches to reduce the morbidity associated with fascioliasis and thus improve the health status of the people.

Keywords


Blood survey; Central Vietnam fascioliasis; F. hepatica; F. gigantica; KAP; prevalence; risk factors

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Keiser, J. and I. Utzinger, Food-borne trematodiasis. Clinical Miriobiological Review, 2009. 22: p. 466-483.

Ozturhan, H., et al., Seroepidemiology of Fasciola Hepatica in Mersin province and surrounding towns and the role of family history of the Fascioliasis in the transmission of the parasite. The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology, 2009. 20(3): p. 198-203.

Sakru, N., et al., Fasciola hepatica Infection in echinococcosis suspected cases. Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2011. 35(2): p. 77.

Walker, S.M., et al., The distribution of Fasciola hepatica and Fasciola gigantica within southern Tanzania - constraints associated with the intermediate host. Parasitology, 2008. 135(4): p. 495-503.

Sothoeun, S., H. Davun, and B. Copeman, Abattoir study on Fasciola gigantica in Cambodian cattle. Tropical Animal Health and Production, 2006. 38(2): p. 113-5.

Mas-Coma, S., Epidemiology of fascioliasis in human endemic areas. Journal of Helminthology, 2005a. 79(3): p. 207-216.

A Marcos, L., et al., Hepatic fibrosis and Fasciola hepatica infection in cattle. Journal of Helminthology, 2007. 81(4): p. 381-6.

Maha, F.M.S., Epidemiological review of human and animal fascioliasis in Egypt. Journal of Infection in Developing Countries, 2008. 2(3): p. 182-189.

Mas-Coma, S., Human fascioliasis: Epidemiological patterns in human endemic areas of South America, Africa and Asia. Southeast Asian Journal of Tropical Medicine and Public Health, 2004b. 35 (Suppl 1): p. 1-11.

Mas-Coma, S., Epidemiology of fascioliasis in human endemic areas. Journal of Helminthology, 2005. 79(3): p. 207-216.

Taraschewski, H., Hosts and parasites as aliens. Journal of Helminthology, 2006. 80(2): p. 99-128.

Kenyon, F., et al., Sheep helminth parasitic disease in south eastern Scotland arising as a possible consequence of climate change. Veterinary parasitology, 2009. 163(4): p. 293-297.

Bargues, M.D., et al., Molecular characterisation of Galba truncatula, Lymnaea neotropica and L. schirazensis from Cajamarca, Peru and their potential role in transmission of human and animal fascioliasis. Parasites & vectors, 2012. 5(1): p. 174-174.

Mas-Coma, S., M.D. Bargues, and M.A. Valero, Fascioliasis and other plant-borne trematode zoonoses. International journal for parasitology, 2005b. 35: p. 1255-1278.

Mas-Coma, S., M.A. Valero, and M.D. Bargues, Fasciola, Lymnaeids and Human Fascioliasis, with a Global Overview on Disease Transmission, Epidemiology, Evolutionary Genetics, Molecular Epidemiology and Control, in Advances in Parasitology Vol 69, D. Rollinson and S.I. Hay, Editors. 2009b, Elsevier Academic Press Inc: San Diego. p. 41-+.

Molyneux, D.H., Control of human parasitic diseases: Context and overview, 2006, ELSEVIER ACADEMIC PRESS INC: SAN DIEGO. p. 1-45.

Mas-Coma, S., The Importance of Emerging and Re-emerging Zoonotic Diseases: Recognition, Monitoring and Control, in FAO/IAEA International Symposium on Sustainable Improvement of Animal Production and Health, N.E.G. Odongo, M.;Viljoen, G. J. , Editor 2010, Food and Agriculture Organization of the United Nations, Rome.: Vienna, Austria. p. 277-284.

Fürst, T., J. Keiser, and J. Utzinger, Global burden of human food-borne trematodiasis: a systematic review and meta-analysis. The Lancet infectious diseases, 2012. 12(3): p. 210-221.

Mas-Coma, S., M.A. Valero, and M.D. Bargues, Fasciola, Lymnaeids and Human Fascioliasis, with a Global Overview on Disease Transmission, Epidemiology, Evolutionary Genetics, Molecular Epidemiology and Control, in Advances in Parasitology Vol 69, D. Rollinson and S.I. Hay, Editors. 2009, Elsevier Academic Press Inc: San Diego. p. 41-+.

WHO, Triclabendazole and fascioliasis - A new drug to combat an age-old disease. . Fact sheet No. 191, 1998: p. 3 pages.

WHO, The "Neglected" Worms. Action Against Worms. 2007a.

Chen, J.-X., et al., An Outbreak of Human Fascioliasis gigantica in Southwest China. . PLoS ONE, 2013. 8(8).

Alawa, C.B.I., et al., In vivo efficacy of Vernonia amygdalina (compositae) against natural helminth infection in Bunaji (Bos indicus) calves. Pakistan Vet. J., 2010. 30: p. 215-218.

Charlier, J., et al., Associations between anti-fasciola hepatica antibody levels in bulk tank milk samples and production parameters in dairy heards. Preventive Veterinary Medicine, 2007. 78: p. 57-66.

Schweitzer, G., et al., Estimating the financial losses due to bovine fascioliasis in Switzerland. Veterinary Record, 2005. 157: p. 188-193.

Rioux, M.C., et al., Discovery and validation of serum biomarkers expressed over the first twelve weeks of Fasciola hepatica infection in sheep. International journal for parasitology, 2008. 38(1): p. 123-136.

Gray, G.D., R.S. Copland, and D.B. Copeman, Overcoming liver fluke as a constraint to ruminant production in South-East Asia, A.C.f.I.A. Research, Editor 2008, Australian Government: Canberra.

Nguyen, M.H., Workshop on reviewing malaria and helminthiasis control activities during 2006-2010 and planning for 2012, 2011, National Institute of Malariology-Parasitology and Entomology. p. 109-111.

Trieu, N.T., Annual workshop reviewing control activities of malaria, parasitic and vector-borne diseases in Central Vietnam in 2010, 2011, Institute of Malariology-Parasitology and Entomology, Quy Nhon. p. 7.

Huynh, H.Q., N.T. Trieu, and V.C. Nguyen, Effectiveness of the therapeutic regiment with triclabendazole on Fasciola gigantica infected patients in Central Vietnam from 2004-2006. Monograph of Scientific research studies, Instiute of Malariology-Parasitology and Entomology, Quy Nhon, 2007: p. 433-443.

Huynh, H.Q., N.T. Trieu, and V.C. Nguyen, Effectiveness and absortion of triclabendazole on Fasciola gigantica and preliminary use of metronidazole for treatment failure cases with triclabendazol in Central Vietnam from 2004-2006. Monograph of Scientific research studies, Instiute of Malariology-Parasitology and Entomology, Quy Nhon, 2007a: p. 444-456.

Nguyen, V.D., et al., Preliminary surveys of fascioliasis in Khanh Hoa province. Journal of Practical Medicine, 2003. 447: p. 125-128.

Nguyen, V.C., V.K. Nguyen, and V.T. Bui, Study on some epidemiological characteristics of fascioliasis in some areas of two provinces in Central Vietnam. Monograph of Scientific research studies (2001-2006). Institute of Malariology-Parasitology and Entomology, Quy Nhon, 2008.

GSO. Statistical data: 1. Administrative Unit and Climate. 2013 [cited 2013 June 22, 2015]; Available from: http://www.gso.gov.vn/default_en.aspx?tabid=773.

Nguyen, V.C., et al., Study on some epidemiological characteristics of fasioliasis in some study sites of Central Vietnam. Monograph of Scientific research studies, 2007: p. 410-416.

MOH, Guidelines on diagnosis and treatment of fascioliasis in humans, Departmen of Treatment, Editor 2006, Ministry of Health: Hanoi.

WHO, Report of the WHO Informal Meeting on use of triclabendazole in fascioliasis control. 2007b.

De, N.V., et al., Evaluation of triclabendazole in fascioliasis treatment in Vietnam in National Institute of Malariology-Parasitology and Entomology. National workshop on Malariology-Parasitology and Entomology during 2001-2005, NIMPE, Editor 2006, NIMPE: Ha Noi. p. 45-53.

Trieu, N.T., Annual workshop on malaria and helminthiasis control in 2005, planning for activities in 2006, 2006, Institute of Malariology-Parasitology and Entomology, Quy Nhon: Quy Nhon.

Tran, V.H. and T.K.D. Tran, Test kits for diagnoses of parasitological diseases. Journal of Practical Medicine, MOH, Viet Nam, 2004. 447: p. 112-116.

Lemeshow, S., et al., Adequacy of sample size in health studies1990, Chichester [England]: Published on behalf of the World Health Organization by Wiley.

Nguyen, V.D. and T.H. Le, Human fascioliasis and Fasciola species in Vietnam. Monograph of Scientific research studies, Instiute of Malariology-Parasitology and Entomology, Quy Nhon, 2007: p. 474-482.

Dang, T.C.T., Fascioliasis in Vietnam and suggested control measures. Journal of Malaria and Parasitology, 2007. 781: p. 129-131.

Dang, T.C.T. and M. To, Clinical, para-clinical characteristics and effectiveness of triclabendazole for the treatment of fascioliasis in patients admitted at North Quang Nam regional general hospital, 2006-2009 Journal of Malaria and Parasitology, 2010. 4: p. 45-55.

Nguyen, K.L., Study on some characteristics of fascioliasis (Fasciola spp.) and effectiveness of intervention measures in Dai Loc district, Quang Nam province, 2010, Vietnam Military Medical University: Ha Noi.

Bland, J.M. and D.G. Altman, Multiple Significance Tests - The Bonferroni Method.10. British Medical Journal, 1995. 310(6973): p. 170-170.

Chadha, V.K., Sample size determination in health studies. NTI Bulletin, 2006. 42(3-4): p. 55-62.

Nguyen, V.C., et al., Trial model of fascioliasis control in two communes of Phu Cat district, Binh Dinh province, Vietnam. Journal of Practical Medicine, 2011. 796: p. 152-156.

Nguyen, V.Q., et al., Animal infection of fasioliasis in Binh Dinh province and preventive and control measures Journal of Science and Technology, Department of Science and Technology, 2010.

Dang, N.T., Morphological identification of fress water invertebrates in Northern Vietnam. Journal of Science, Hanoi-Vietnam, 1980: p. 440-490.

Pham, N.D. and T.L. Nguyen, Characteristics of identifying cercaria of trematoda and differentiation of cercaria in Fasciola gigantica Cobbold 1885 in Lymnaid snails in Vietnam Journal of Biology-Vietnam National Centre for Natural Science and Technology, 2005. 27(3A): p. 31-36.

Nguyen, V.V., Study on human fascioliasis and effectiveness of intervention measures in a community of Quang Nam during 2009-2011., in Parasitology Department2012, National Institute of Malariology-Parasitology and Entomology: Ha Noi.

Nguyen, V.K., Prevalence of fascioliasis in some study sites of two central provinces of Quang Ngai and Gia Lai. Monograph of Scientific and Technological Research 2001-2006, 2007(Institute of Malariology-Parasitology and Entomology, Quy Nhon): p. 462-465.

Nguyen, X.M., Study on prevalence and related factors of human infection in some plain areas of Quang Ngai province, 2009, Department of Science and Technology-Quang Ngai province: Quang Ngai.

Tran, V.H., et al., Infection rates of Fasciola sp. in two communes Chu Pa and H'Bong of Gia Lai province Journal of Military Pharmaco-Medicine - Vietnam Military Medical University, 2008. 2: p. 75-78.

Nguyen, T.H., Study on some characteristics and treatment effectiveness of fascioliasis with triclabendazole in Tinh Ky and Nghia Son communes, Quang Ngai province, 2012, National Institute of Malariology-Parasitology and Entomology: Ha Noi. p. 163.

Mas-Coma, S., J.G. Esteban, and M.D. Bargues, Epidemiology of human fascioliasis: a review and proposed new classification. Bulletin of the World Health Organization, 1999a. 77: p. 340-346.

Ashrafi, K., et al., Plant- borne human contamination by fascioliasis. Am. J. Trop. Med. Hyg., 2006. 75(2): p. 295-302.

Le, T.H., et al., Human fascioliasis and the presence of hybrid/introgressed forms of Fasciola hepatica and Fasciola gigantica in Vietnam. International journal for parasitology, 2008. 38(6): p. 725-730.

Das, K., et al., Non-resolving liver abscess with Echinococcus cross-reactivity in a non-endemic region. Indian Journal of Gastroenterology, 2007. 26(2): p. 92-93.

Ramzy, R.M.R., et al., An enzyme‐linked immunosorbent assay for detection of IgG1 antibodies specific to human cystic echinococcosis in Egypt. Tropical Medicine & International Health, 1999. 4(9): p. 616-620.

Nguyen, K.L., N.A. Do, and N.S. Nguyen, Identification of fascioliasis infection in cattle and risk factors of human transmission in Dai Loc district-Quang Nam province. Jounal of Malaria, Parasitic and Entomological control-National Institute of Malariology-Parasitology and Entomology, 2011. 3: p. 76-82.

Kumar, N., S. Ghosh, and S.C. Gupta, Early detection of Fasciola gigantica infection in buffaloes by enzyme-linked immunosorbent assay and dot enzyme-linked immunosorbent assay. Parasitology research, 2008. 103(1): p. 141-150.

Rokni, M.B., J. Massoud, and A. Hanilo, Comparison of adult somatic and cysteine proteinase antigens of Fasciola gigantica in enzyme linked immunosorbent assay for serodiagnosis of human fasciolosis. Acta Tropica, 2003. 88(1): p. 69-75.

Reichel, M.P., Performance characteristics of an enzyme-linked immunosorbent assay for the detection of liver fluke ( Fasciola hepatica) infection in sheep and cattle. Veterinary parasitology, 2002. 107(1): p. 65-72.

Apt, W., X. Aguilera, and F. Vega, Treatment of human chronic fascioliasis with triclabendazole: drug efficacy and serologic response. Am. J. Trop. Med. Hyg., 1995. 52: p. 532-535.

Espinoza, J.R., et al., EVALUATION OF FAS2-ELISA FOR THE SEROLOGICAL DETECTION OF FASCIOLA HEPATICA INFECTION IN HUMANS. American Journal of Tropical Medicine and Hygiene, 2007. 76(5): p. 977-982.

Huynh, H.Q., et al., Reviews on animal husbandry and awareness of community on fascioliasis in two Central provinces of Quang Nam and Quang Ngai. Monograph of Scientific research studies, Instiute of Malariology-Parasitology and Entomology, Quy Nhon, 2011.

David, A., et al., Immunoglobulin E and Eosinophil-Dependent Protective Immunity to Larval Onchocerca volvulus in Mice Immunized with Irradiated Larvae. Infection and Immunity, 2004. 72(2): p. 810-817.

Meeusen, E.N.T. and A. Balic, Do Eosinophils have a Role in the Killing of Helminth Parasites?, 2000, Elsevier B.V: ENGLAND. p. 95-101.

Aly, I.R., et al., Fasciola gigantica Fatty Acid Binding Protein (FABP) as a Prophylactic Agent against Schistosoma mansoni Infection in CD1 Mice. The Korean Journal of Parasitology, 2012. 50(1): p. 37-43.

Nguyen, K.L., T.C.T. Dang, and M. To, Fascioliasis infection in Dai Loc district, Quang Nam province and effectiveness of community-based control measreas. Journal of Military Pharmaco-Medicine - Vietnam Military Medical University, 2008. 33(2): p. 71-74.

Curtale, F., et al., Human fascioliasis infection: gender differences within school-age children from endemic areas of the Nile Delta, Egypt. Transactions of the Royal Society of Tropical Medicine and Hygiene, 2007. 101(2): p. 155-160.

Curtale, F., et al., Clinical signs and household characteristics associated with human fascioliasis among rural population in Egypt: A case-control study. Parassitologia, 2003. 45(1): p. 5-11.

Esteban, J.G., et al., High endemicity of human fascioliasis between Lake Titicaca and La Paz valley, Bolivia. Transactions of the Royal Society of Tropical Medicine and Hygiene, 1999. 93: p. 151-156.

Esteban, J.G., et al., High fascioliasis infection in children linked to a man-made irrigation zone in Peru. Tropical Medicine & International Health, 2002. 93: p. 151-156.

Phan, G.C., K.S. Pham, and D. Nguyen, Some reviews on fascioliasis in the first 9 months of 2008 in Gia Lai province. Monograph of Scientific research studies, National Instiute of Malariology-Parasitology and Entomology, Hanoi, 2008. 6: p. 82-87.

Nguyen, T.G., et al., Bovine fasciolosis in the human fasciolosis hyperendemic Binh Dinh province in Central Vietnam. Acta tropica, 2011. 117(1): p. 19-22.

Nguyen, S.T., et al., Prevalence of Fasciola in cattle and of its intermediate host Lymnaea snails in central Vietnam. Tropical Animal Health and Production, 2012. 44(8): p. 1847-1853.

Marcos, L., et al., Risk factors for Fasciola hepatica infection in children: a case-control study. Transactions of the Royal Society of Tropical Medicine and Hygiene, 2006. 100(2): p. 158-166.

Rojas, L., et al., Fascioliasis: can Cuba conquer this emerging parasitosis? Trends in parasitology, 2010. 26(1): p. 26-34.

Muhsin Kaya Remzi Bestas Sedat, C., Clinical presentation and management of Fasciola hepatica infection：Single-center experience. 世界胃肠病学杂志：英文版, 2011. 17(44): p. 4899-4904.

Aroonroch, R., et al., Hepatic fascioliasis due to Fasciola hepatica: a two-case report. Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 2006. 89(10): p. 1770.

Zhou, X.-N., Prioritizing research for "One health - One world". Infectious diseases of poverty, 2012. 1(1): p. 1-1.

Agnamey, P., et al., Cross-sectional serological survey of human fascioliasis in haiti. Journal of parasitology research, 2012. 2012(Journal Article): p. 751951-3.

Tolan, R.W., Fascioliasis Due to Fasciola hepatica and Fasciola gigantica Infection: An Update on This 'Neglected' Neglected Tropical Disease. LABMEDICINE, 2011. 42(2): p. 107-116.

Crompton, D.W.T. and L. Savioli, Handbook of Helminthiasis for Public Health2006, Boca Raton, FL. USA: Taylor & Francis Group.

Dang, T.C.T., et al., Epidemiological charateristics and control measures of fascioliasis in Vietnam N.P. Works, Editor 2011, Ministry of Health: Hanoi.

Dang, T.T. and Y. Nawa, Fasciola and fascioliasis in Vietnam. Asia Parasitology, 2005. 1: p. 57-60.